scRNA-seq assessment of the human lung, spleen, and esophagus tissue stability after cold preservation
Publication: Genome Biology
E. Madissoon, A. Wilbrey-Clark, R. J. Miragaia, K. Saeb-Parsy, K. T. Mahbubani, N. Georgakopoulos, P. Harding, K. Polanski, N. Huang, K. Nowicki-Osuch, R. C. Fitzgerald, K. W. Loudon, J. R. Ferdinand, M. R. Clatworthy, A. Tsingene, S. van Dongen, M. Dabrowska, M. Patel,
M. J. T. Stubbington, S. A. Teichmann, O. Stegle & K. B. Meyer
31 December 2019
Summary:
The Human Cell Atlas, which is an international collaboration to map all the cell types in the human body.
However, delays between fresh sample collection and processing may lead to poor data and difficulties in experimental design.
This study looked at the effect of cold storage on fresh healthy spleen, esophagus, and lung from donors and concluded that cold storage of tissue works well and increases the time frame for processing samples; however robust protocols are needed for tissue preservation that can be used by all the research teams involved.